METHOD FOR PRODUCING SIALIC ACID-CONTAINING SUGAR CHAIN Russian patent published in 2016 - IPC C12P19/18 C12P19/04 C08B37/00 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology. Disclosed are methods of producing sialylated sugar chain. Method comprises reacting a sugar chain with CMP-sialic acid in presence of ST6Gal-I and phosphatase temperature from 20 °C to 37 °C for 8-48 hours to produce triantennary or tetraantennary N-linked sialylated sugar chain, containing sialic acid on each of its non-reducing ends. In another version, before said step, method involves reaction of sugar chain with UDP sugar in presence of glycosyltransferase. In third version, method involves reaction of agalacto-biantennary complex sugar chain with UDP-GlcNAc in presence of MGAT4 and MGAT5. Obtained product reacts with UDP-Gal in presence of β4GalT1, then with CMP-sialic acid in presence of ST6Gal-I and phosphatase.

EFFECT: invention enables to obtain triantennary or tetraantennaryα2,6-sialylated on each of their non-reducing ends sugar chains with high output in single-reactor synthesis.

15 cl, 9 dwg