FIELD: biochemistry.
SUBSTANCE: described is a method of producing a protein of interest, involving introduction of at least one expression vector, which contains gene fragment containing DNA coding protein, and also a pair of transposon sequences Tol1 or Tol2 on both ends of a gene fragment in a mammal cell suspension CHO and suspension culturing of mammal cells, where mammal cell suspension is a CHO cell suspension, which is capable of surviving and proliferating in serum-free medium. Invention also discloses corresponding suspension cell and a vector.
EFFECT: invention allows for short period of time effective and high level of producing protein.
31 cl, 13 dwg, 11 tbl, 13 ex
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Year |
Author |
Number |
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2010 |
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|
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2020 |
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|
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2019 |
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|
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2020 |
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|
RU2817416C2 |
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2017 |
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|
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2008 |
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|
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2023 |
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2015 |
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|
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2014 |
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|
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2023 |
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|
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