FIELD: biotechnology.
SUBSTANCE: present invention relates to methods of differentiation of pluripotent stem cells. In particular present invention discloses methods for characterizing cells, differentiated into cells, expressing markers, characteristic for pancreatic endocrine cell line, based on analysis of unique cell surface markers. Present invention also discloses methods of enriching or sorting cells, expressing markers, characteristic for pancreatic endocrine cell line.
EFFECT: present invention also discloses methods of reducing number of cells, which can be harmful for cell population, expressing markers, characteristic for pancreatic endocrine cell line, formed by methods, which are subject of this invention, thereby reducing rate of tumors in vivo after transplantation.
13 cl, 13 dwg, 7 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS FOR PURIFYING CELLS DERIVED FROM PLURIPOTENT STEM CELLS | 2011 |
|
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| METHODS OF PRODUCING CELLS OF DEFINITIVE AND PANCREATIC ENDODERM | 2013 |
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RU2668814C2 |
| DIFFERENTIATION OF HUMAN EMBRYO STEM CELLS | 2011 |
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RU2587634C2 |
| DIFFERENTIATION OF HUMAN EMBRYO STEM CELLS | 2011 |
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| DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS | 2022 |
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RU2805433C1 |
| DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS | 2018 |
|
RU2782271C2 |
| TREATMENT OF DIABETES WITH HELP OF PANCREATIC ENDOCRINE PROGENITOR CELLS | 2011 |
|
RU2682719C2 |
| DIFFERENTIATION OF PLURIPOTENT STEM CELLS | 2011 |
|
RU2599420C2 |
| METHOD FOR OBTAINING PANCREATIC ENDOCRINE CELLS (VERSIONS) AND METHOD FOR INCREASING OUTPUT OF BETA CELLS | 2013 |
|
RU2684215C2 |
| DIFFERENTIATION OF PLURIPOTENT STEM CELLS | 2011 |
|
RU2673946C1 |
