FIELD: biotechnology; medicine.
SUBSTANCE: invention relates to biotechnology and medicine, particularly to methods for stimulating differentiation of pluripotent stem cells into insulin-producing cells. Invention provides a method for producing a population of cells expressing markers specific for a pancreatic endoderm line, wherein more than 80% of cells in the population simultaneously express PDX1 and NKX6.1, as well as the population itself.
EFFECT: invention represents an alternative approach to increasing the efficiency of differentiation of human embryonic stem cells into insulin-expressing cells.
22 cl, 5 dwg, 2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS | 2018 |
|
RU2782271C2 |
| DIFFERENTIATION OF HUMAN EMBRYO STEM CELLS | 2011 |
|
RU2663339C1 |
| DIFFERENTIATION OF HUMAN EMBRYO STEM CELLS | 2011 |
|
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| DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS | 2022 |
|
RU2805433C1 |
| DIFFERENTIATION OF PLURIPOTENT STEM CELLS | 2011 |
|
RU2599420C2 |
| METHOD FOR OBTAINING PANCREATIC ENDOCRINE CELLS (VERSIONS) AND METHOD FOR INCREASING OUTPUT OF BETA CELLS | 2013 |
|
RU2684215C2 |
| DIFFERENTIATION OF PLURIPOTENT STEM CELLS | 2011 |
|
RU2673946C1 |
| DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS | 2010 |
|
RU2610176C2 |
| METHOD FOR OBTAINING CELLS EXPRESSING MARKERS CHARACTERISTIC FOR PANCREATIC ENDOCRINE CELLS | 2013 |
|
RU2658488C2 |
| METHOD FOR PRODUCING POPULATION OF PANCREATIC ENDOCRINE CELLS CO-EXPRESSING NKX6.1 AND INSULIN, AND METHOD OF TREATING DIABETES | 2010 |
|
RU2701335C2 |
