HUMANIZED IL-6 AND IL-6 RECEPTOR Russian patent published in 2017 - IPC A01K67/27 C07K14/54 C07K14/715 C12N15/00 C12N15/90 

FIELD: biotechnology.

SUBSTANCE: invention relates to genetically modified mouse family animals, namely, to mice and rats that express human IL-6 and may further express humanized IL-6Rα. This animal carries the replacement of its gene encoding IL-6 at its endogenous locus IL-6 with the human gene encoding human IL-6 and further replacement of the sequence encoding animal extracellular domain IL-6Rα at its endogenous locus IL-6Rα, with the sequence encoding the humen extracellular domain IL-6Rα to produce a humanized IL-6Rα gene. At that, the human gene encoding human IL-6 and humanized IL-6Rα gene are controlled by the endogenous regulatory elements of the animal at its endogenous locus IL-6 and IL-6Rα respectively. This humanized IL-6Rα gene contains endogenous transmembrane and cytoplasmic sequences of IL-6Rα of the said animal. The invention also discloses a method for obtaining of these animals and isolated embryonic stem cells for obtaining of such animals.

EFFECT: invention allows to obtain transgenic mice and rats without any pathology.

18 cl, 15 dwg, 3 tbl, 3 ex