METHOD OF DIAGNOSTICS OF PREECLAMPSIA ON THE GESTATION UP TO 34 WEEK BY EVALUATION OF LEVELS OF ANG (1-8) VASOCONSTRICTOR AND ANG (1-7) VASODILATOR Russian patent published in 2018 - IPC G01N33/00 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to obstetrics and gynecology, and can be used to diagnose preeclampsia at terms up to 34 weeks gestation. Method includes evaluation of the levels of the angiotensin II vasoconstrictor (ANG (1-8)) and angiotensin 1-7 vasodilator (ANG (1-7)). By means of an enzyme-linked immunosorbent assay (ELISA) on plasma samples, determine the concentration of the ANG vasodilator (1-7) and the ANG vasoconstrictor (1-8), as well as the ratio ANG (1-7)/ANG (1-8). Determination of markers is carried out by quantitative enzyme-linked immunosorbent assay using Angiotensin (ANG 1-7) and Angiotensin (ANG 1-8) sets on blood plasma samples. Blood plasma (0.2 ml) is obtained from whole blood taken from the ulnar vein, 3 ml in volume, into a tube with EDTA, centrifuged at 3,000 rpm for 20 minutes. If there is a concentration of the ANG (1-7) vasodilator less than 500 pg/ml and with a ratio ANG (1-7)/ANG (1-8) less than 25, diagnose preeclampsia.

EFFECT: use of the invention makes it possible to increase the accuracy of diagnosis, to reduce the incidence of adverse outcomes in this disease.

1 cl, 3 ex