FIELD: cell biology; medicine.
SUBSTANCE: present invention relates to a method of producing cells expressing markers characteristic of pancreatic endodermal cells, and cells expressing markers characteristic of pancreatic endocrine precursor cells by differentiation. To implement the method, cells expressing markers characteristic of pancreatic progenitor cells of the foregut, are treated with an ALK5 inhibitor, or a thyroid hormone selected from triiodothyronine, thyroxine, triiodothyronine analogues, thyroxine analogues or a mixture thereof, or simultaneously an ALK5 inhibitor and a thyroid hormone. Further, the cells are cultured in an air-liquid interaction zone.
EFFECT: present invention widens the range of methods for producing functional endocrine cells and, in particular, functional beta cells.
23 cl, 23 dwg, 13 tbl, 14 ex
