FIELD: biotechnology.
SUBSTANCE: group of inventions relates to the field of biotechnology, genetic engineering and medicine. Proposed artificial gene YkuJ-MPER encodes a protein-immunogen YkuJ-MPER against HIV-1 with the nucleotide sequence of SEQ ID NO:1 of length of 360 bps. Recombinant plasmid DNA of pET21a-YkuJ-MPER type is also proposed, designed to produce a protein-immunogen that induces HIV-specific B-cell response, with a size of 5,700 bps and a molecular weight of 3.8·103 kDa containing the YkuJ-MPER gene, encoding the YkuJ-MPER anti-HIV-1 protein-immunogen, under the control of the RNA polymerase promoter of phage T7, ensuring its expression in E. coli cells. Artificial YkuJ-MPER immunogen protein is also proposed, consisting of a B. subtilis YkuJ protein site and two HIV-1 MPER sites, having a molecular weight of 14.2 kDa and the amino acid sequence of SEQ ID NO:2.
EFFECT: group of inventions provides an effective induction of broadly neutralizing anti-HIV-1 antibodies in the selection of the optimal immunization strategy.
3 cl, 12 dwg, 1 tbl, 6 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT CHIMERICAL nTBI POLYPEPTIDE-IMMUNOGEN WITH ABILITY TO INDUCE NEUTRALIZING ANTIBODIES TO TYPE 1 HUMAN IMMUNODEFICIENCY VIRUS AND INTENDED FOR USE AS COMPONENT OF VACCINE AGAINST HIV-1 |
2016 |
- Chikaev Anton Nikolaevich
- Karpenko Larisa Ivanovna
- Rudometov Andrej Pavlovich
- Andreeva Nadezhda Borisovna
- Ilichev Aleksandr Alekseevich
- Shcherbakova Nadezhda Sergeevna
|
RU2642258C1 |
| ARTIFICIAL PROTEIN-IMMUNOGEN TCI COMPRISING MULTIPLE CTL-EPITOPES OF HIV-1 BASIC ANTIGENS, ARTIFICIAL GENE TCI ENCODING POLYEPITOPE PROTEIN-IMMUNOGEN TCI |
2002 |
- Bazhan S.I.
- Belavin P.A.
- Seregin S.V.
- Babkina I.N.
- Daniljuk N.K.
- Sandakhchiev L.S.
|
RU2238946C2 |
| CR3 CHIMERA GENE AND CR3 CHIMERA PROTEIN ENCODED THEREWITH (VARIANTS) INDUCING ANTI-HIV-1 IMMUNE RESPONSE |
2002 |
- Iglesias Peres Ehnrike
- Vaskes Blomkist Danija M.
- Duarte Kano Karlos A.
|
RU2302461C2 |
| RECOMBINANT PLASMID DNA PTBI-HBSAG COMPRISING CHIMERIC GENE TBI-HBSAG UNDER CONTROL OF SMALLPOX VACCINE PROMOTER P7,5K AND STRAIN OF RECOMBINANT SMALLPOX VACCINE INDUCING IMMUNE RESPONSE AGAINST HIV AND HUMAN HEPATITIS B IN ANIMAL BODY |
2001 |
- Shchelkunov S.N.
- Rjazankin I.A.
- Ageenko V.A.
- Ignat'Ev G.M.
- Babkin I.V.
|
RU2194075C2 |
| MEL-TCI-A0201 ARTIFICIAL GENE CODING MEL-TCI-A0201 POLYEPITOPE IMMUNOGENIC PROTEIN, RECOMBINANT PLASMID DNA OF pMEL-TCI-A0201, PROVIDING EXPRESSION OF MEL-TCI-A0201 ARTIFICIAL GENE AND MEL-TCI-A0201 ARTIFICIAL IMMUNOGENIC PROTEIN CONTAINING MULTIPLE CTL- AND Th-EPITOPES OF MELANOMA ANTIGENS |
2012 |
- Antonets Denis Viktorovich
- Bazhan Sergej Ivanovich
- Il'Ichev Aleksandr Alekseevich
- Karpenko Larisa Ivanovna
- Borobova Elena Aleksandrovna
- Starostina Ekaterina Vladimirovna
- Smirnova Ol'Ga Jur'Evna
- Oreshkova Svetlana Fedorovna
|
RU2522830C2 |
| ARTIFICIAL GENES CODING EV.CTL AND EV.Th PROTEINS-IMMUNOGENS, RECOMBINANT PLASMID DNA pEV.CTL AND EV.Th, PROVIDING EXPRESSION OF ARTIFICIAL GENES, AND ARTIFICIAL T-CELL POLYEPITOPE PROTEINS-IMMUNOGENS EV.CTL AND EV.Th CONTAINING EPITOPES OF EBOLA VIRUS ANTIGENS, USED TO CREATE AN EBOLA VIRUS VACCINE |
2018 |
- Antonets Denis Viktorovich
- Bazhan Sergej Ivanovich
- Ilichev Aleksandr Alekseevich
- Kaplina Olga Nikolaevna
- Karpenko Larisa Ivanovna
- Smirnova Olga Yurevna
- Oreshkova Svetlana Fedorovna
|
RU2713723C1 |
| ARTIFICIAL GENE ENCODING THE BSI-COV IMMUNOGEN PROTEIN, PBSI-COV RECOMBINANT PLASMID DNA PROVIDING EXPRESSION OF THE TARGET GENE, AND AN ARTIFICIAL BSI-COV POLYEPITOPE IMMUNOGEN PROTEIN CONTAINING EPITOPES OF SARS-CoV-2 VIRUS ANTIGENS AND INDUCING SARS-CoV-2- SPECIFIC T-CELL IMMUNITY |
2023 |
- Bazhan Sergej Ivanovich
- Borgoyakova Mariya Borisovna
- Karpenko Larisa Ivanovna
- Rudometov Andrej Pavlovich
- Starostina Ekaterina Vladimirovna
- Ilichev Aleksandr Alekseevich
|
RU2806556C1 |
| ARTIFICIAL GENE ENCODING THE BSI-COV-UB IMMUNOGEN PROTEIN, RECOMBINANT PLASMID DNA PBSI-COV-UB, WHICH ENSURES THE EXPRESSION OF THE TARGET GENE, AND AN ARTIFICIAL POLYEPITOPE BSI-COV-UB IMMUNOGEN PROTEIN CONTAINING UBIQUITIN AND EPITOPES OF SARS-CoV-2 VIRUS ANTIGENS AND INDUCING SARS-CoV-2-SPECIFIC T-CELL IMMUNITY |
2023 |
- Bazhan Sergej Ivanovich
- Borgoyakova Mariya Borisovna
- Karpenko Larisa Ivanovna
- Rudometov Andrej Pavlovich
- Starostina Ekaterina Vladimirovna
- Ilichev Aleksandr Alekseevich
|
RU2806590C1 |
| RECOMBINANT VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 |
2006 |
- Karpenko Larisa Ivanovna
- Bazhan Sergej Ivanovich
- Lebedev Leonid Rudol'Fovich
- Nekrasova Nadezhda Aleksandrovna
- Belavin Pavel Aleksandrovich
- Seregin Sergej Viktorovich
- Daniljuk Nadezhda Konstantinovna
- Eroshkin Aleksej Maksimovich
- Il'Ichev Aleksandr Alekseevich
|
RU2317107C2 |
| RECOMBINANT PLASMID DNA PCDNA-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE TCI IN EUCARYOTIC CELLS AND RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERITIDIS E-23 PC-DNA-TCI AS CANDIDATE FOR CONSTRUCTION OF LIVE DNA-VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS |
2003 |
- Karpenko L.I.
- Bazhan S.I.
- Nekrasova N.A.
- Belavin P.A.
- Daniljuk N.K.
- Seregin S.V.
- Babkina I.N.
- Ignat'Ev G.M.
- Agafonov A.P.
- Bojchenko M.N.
- Vorob'Ev A.A.
- Il'Ichev A.A.
- Sandakhchiev L.S.
|
RU2248396C2 |
