FIELD: biotechnology.
SUBSTANCE: group of inventions relates to the field of biotechnology, genetic engineering and medicine. Proposed artificial gene YkuJ-MPER encodes a protein-immunogen YkuJ-MPER against HIV-1 with the nucleotide sequence of SEQ ID NO:1 of length of 360 bps. Recombinant plasmid DNA of pET21a-YkuJ-MPER type is also proposed, designed to produce a protein-immunogen that induces HIV-specific B-cell response, with a size of 5,700 bps and a molecular weight of 3.8·103 kDa containing the YkuJ-MPER gene, encoding the YkuJ-MPER anti-HIV-1 protein-immunogen, under the control of the RNA polymerase promoter of phage T7, ensuring its expression in E. coli cells. Artificial YkuJ-MPER immunogen protein is also proposed, consisting of a B. subtilis YkuJ protein site and two HIV-1 MPER sites, having a molecular weight of 14.2 kDa and the amino acid sequence of SEQ ID NO:2.
EFFECT: group of inventions provides an effective induction of broadly neutralizing anti-HIV-1 antibodies in the selection of the optimal immunization strategy.
3 cl, 12 dwg, 1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT CHIMERICAL nTBI POLYPEPTIDE-IMMUNOGEN WITH ABILITY TO INDUCE NEUTRALIZING ANTIBODIES TO TYPE 1 HUMAN IMMUNODEFICIENCY VIRUS AND INTENDED FOR USE AS COMPONENT OF VACCINE AGAINST HIV-1 | 2016 |
|
RU2642258C1 |
| ARTIFICIAL PROTEIN-IMMUNOGEN TCI COMPRISING MULTIPLE CTL-EPITOPES OF HIV-1 BASIC ANTIGENS, ARTIFICIAL GENE TCI ENCODING POLYEPITOPE PROTEIN-IMMUNOGEN TCI | 2002 |
|
RU2238946C2 |
| CR3 CHIMERA GENE AND CR3 CHIMERA PROTEIN ENCODED THEREWITH (VARIANTS) INDUCING ANTI-HIV-1 IMMUNE RESPONSE | 2002 |
|
RU2302461C2 |
| RECOMBINANT PLASMID DNA PTBI-HBSAG COMPRISING CHIMERIC GENE TBI-HBSAG UNDER CONTROL OF SMALLPOX VACCINE PROMOTER P7,5K AND STRAIN OF RECOMBINANT SMALLPOX VACCINE INDUCING IMMUNE RESPONSE AGAINST HIV AND HUMAN HEPATITIS B IN ANIMAL BODY | 2001 |
|
RU2194075C2 |
| RECOMBINANT PLASMID DNA PCDNA-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE TCI IN EUCARYOTIC CELLS AND RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERITIDIS E-23 PC-DNA-TCI AS CANDIDATE FOR CONSTRUCTION OF LIVE DNA-VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2003 |
|
RU2248396C2 |
| RECOMBINANT VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 | 2006 |
|
RU2317107C2 |
| MEL-TCI-A0201 ARTIFICIAL GENE CODING MEL-TCI-A0201 POLYEPITOPE IMMUNOGENIC PROTEIN, RECOMBINANT PLASMID DNA OF pMEL-TCI-A0201, PROVIDING EXPRESSION OF MEL-TCI-A0201 ARTIFICIAL GENE AND MEL-TCI-A0201 ARTIFICIAL IMMUNOGENIC PROTEIN CONTAINING MULTIPLE CTL- AND Th-EPITOPES OF MELANOMA ANTIGENS | 2012 |
|
RU2522830C2 |
| PEPTIDE IMMUNOGENS AND VACCINE COMPOSITION AGAINST COVID-19 WITH THE USE OF PEPTIDE IMMUNOGENS | 2020 |
|
RU2743593C1 |
| ARTIFICIAL GENES CODING EV.CTL AND EV.Th PROTEINS-IMMUNOGENS, RECOMBINANT PLASMID DNA pEV.CTL AND EV.Th, PROVIDING EXPRESSION OF ARTIFICIAL GENES, AND ARTIFICIAL T-CELL POLYEPITOPE PROTEINS-IMMUNOGENS EV.CTL AND EV.Th CONTAINING EPITOPES OF EBOLA VIRUS ANTIGENS, USED TO CREATE AN EBOLA VIRUS VACCINE | 2018 |
|
RU2713723C1 |
| PEPTIDE IMMUNOGENS AND A VACCINE COMPOSITION AGAINST CORONAVIRUS INFECTION COVID-19 USING PEPTIDE IMMUNOGENS | 2020 |
|
RU2738081C1 |
