METHOD FOR RAPID ANALYSIS OF URINE ON GENES OF BETA-LACTAM RESISTANCE OF UROPATHOGENIC ENTEROBACTERIA IN PREGNANT WOMEN WITH PYELONEPHRITIS Russian patent published in 2020 - IPC G01N33/58 G01N33/493 

Abstract RU 2738854 C1

FIELD: medicine.

SUBSTANCE: invention relates to the field of medicine. What is presented is a urine rapid urine analysis method on genes of beta-lactam resistance of uropathogenic enterobacteria in pregnant women with pyelonephritis. Urine samples are analyzed for DNA of enterobacteria by quantitative PCR in real time and if observing a clinically significant amount of enterobacteria ≥5×105 GE/ml followed by PCR analysis of the same DNA sample on beta-lactamase genes CTX-M, TEM and DHA. If observing at least one of genes CTX-M, TEM, DHA conclusion is made on beta-lactam resistance of uropathogenic enterobacteria.

EFFECT: invention provides reduced time of investigation and possibility of using in institutions that do not have conditions for bacteriological studies, but perform PCR-analysis.

1 cl, 1 dwg, 3 tbl, 3 ex

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RU 2 738 854 C1

Authors

Khusnutdinova Tatyana Alekseevna

Shipitsyna Elena Vasilevna

Shalepo Kira Valentinovna

Krysanova Anna Aleksandrovna

Budilovskaya Olga Viktorovna

Gerasimova Ekaterina Nikolaevna

Savicheva Alevtina Mikhajlovna

Dates

2020-12-17Published

2020-06-26Filed