METHODS AND COMPOSITIONS FOR PURIFICATION OR ISOLATION OF MICROVESICLES AND EXOSOMES Russian patent published in 2021 - IPC G01N1/18 B01D15/38 A61K9/127 C12N5/00 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to the field of biotechnology. A method and device for obtaining a composition or isolate of exosomes or microvesicles are proposed. The method includes providing a liquid that includes exosomes or microvesicles, providing a substrate surface with an array of exosome-binding ligands, interacting the liquid with the substrate in conditions that bind exosomes or microvesicles to exosome-binding ligands, and separating the substrate from the liquid to obtain a composition or isolate of exosomes or microvesicles. At the same time, each ligand is in the form of an electron-rich group at pH 4-8, and an array of exosome-binding ligands allows exosomes or microvesicles to bind to the substrate. The device includes a substrate with the above array of exosome-binding ligands.

EFFECT: inventions provide improved exosome yield, scalability for manufacturing, or obtaining pharmaceutical grade exosomes.

18 cl, 19 dwg, 2 tbl, 14 ex