FIELD: biotechnology; cell biology.
SUBSTANCE: invention relates to biotechnology and cell biology, in particular to a method for producing mesenchymal stem cells (hereinafter – MSC) by differentiating induced pluripotent stem cells (hereinafter – iPSC). To implement the method, a primary mesodermal cell differentiated from iPSC is first cultivated in a mesenchymal colony formation medium (hereinafter – M-CFM) containing LiCl and FGF2, but in the absence of PDGF, under conditions with a normal oxygen content for sufficient time for the formation of a mesenchymal colony. Then, the mesenchymal colony is cultivated in an adhesive state to obtain MSC. The resulting MSC has improved T-cell immunosuppression properties compared to MSC that is not produced in the specified M-CFM. The invention also relates to a method for treating conditions in which an immunosuppressive effect is useful, selected from the following: “graft-versus-host” reaction, systemic lupus erythematosus (SLE), Crohn’s disease, multisystem atrophy, multiple sclerosis, amyotrophic lateral sclerosis, stroke, cardiovascular disorders, orthopedic disorders or rejection of transplanted solid organs.
EFFECT: present invention makes it possible to improve and scale methods for differentiating iPSC in MSC.
13 cl, 20 dwg, 9 tbl, 7 ex
