BACTERIUM WHICH PRODUCES MONOPHOSPHORYL LIPID A, AND A METHOD OF PRODUCING MONOPHOSPHORYL LIPID USING A BACTERIUM Russian patent published in 2020 - IPC C12N15/52 C12N15/63 C12N9/10 C12N9/16 C12P19/04 C12P7/64 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to a recombinant bacterium which produces monophosphoryl lipid A (MLA), not conjugated with 2-keto-3-deoxy-D-manno-octulosonate (Kdo), and a method of producing MLA, not conjugated with Kdo, using said bacterium. Disclosed is a recombinant bacterium producing MLA not conjugated with Kdo, transformed with at least one exogenous polynucleotide, coding polypeptide 1-phosphatase lipid A (LpxE). Said recombinant bacterium is modified by transformation with at least one of exogenous polynucleotide, encoding a polypeptide of a lipoid transferase biosynthesis lipid A (LpxL), an exogenous polynucleotide encoding a lipid A (LpxM) biosynthesis myristoyltransferase polypeptide, or a combination thereof. Said recombinant bacterium exhibits high expression of the polynucleotide encoding the LpxL polypeptide, the polynucleotide encoding the LpxM polypeptide, or a combination thereof as compared to a corresponding recombinant bacterium without said modification, and wherein at least one of the following genes is deleted, or the expression of at least one of the following genes is reduced: a gene encoding a KdtA polypeptide, a gene encoding a KdsB polypeptide, a gene encoding a KdsC polypeptide, a gene encoding a KdsA polypeptide, a gene encoding a GutQ polypeptide, a gene encoding a KpsF polypeptide, a gene encoding a KpsU polypeptide, a gene encoding a KdsD polypeptide, or a combination thereof. What is also presented is a method for producing MLA not conjugated with Kdo, involving culturing said bacteria to produce a culture and isolating MLA not conjugated with Kdo from the culture.

EFFECT: disclosed are a bacterium which produces monophosphoryl lipid A and a method of producing monophosphoryl lipid using a bacterium.

14 cl, 5 dwg, 3 ex