FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to a ribonucleoprotein complex for human genome editing by inserting a sequence of interest into it, which includes the protein spCas9 linked to sgRNA-PP7 with an encoded sequence that is linked to an effector chimeric protein.
EFFECT: invention is effective in modeling human genetic diseases on cellular systems.
1 cl, 4 dwg, 1 tbl
| Title |
Year |
Author |
Number |
| RIBONUCLEOPROTEIN COMPLEX FOR HUMAN GENOME EDITING BY INSERTING SEQUENCE OF INTEREST INTO IT |
2020 |
- Ivanenko Aleksandr Vyacheslavovich
- Belousov Vsevolod Vadimovich
|
RU2750939C1 |
| SYSTEM FOR ATTRACTING PROTEIN TO DNA BREAK SITE TO INCREASE EFFICIENCY OF MAMMALIAN GENOME EDITING |
2022 |
- Ivanenko Aleksandr Vyacheslavovich
- Belousov Vsevolod Vadimovich
|
RU2808601C1 |
| SYSTEM FOR INCREASING EFFICIENCY OF MAMMALIAN GENOME EDITING THROUGH HOMOLOGOUS REPAIR |
2022 |
- Ivanenko Aleksandr Vyacheslavovich
- Belousov Vsevolod Vadimovich
|
RU2808045C1 |
| METHOD OF EDITING MAMMALIAN GENOME BY HOMOLOGOUS REPAIR |
2022 |
- Ivanenko Aleksandr Vyacheslavovich
- Belousov Vsevolod Vadimovich
|
RU2808600C1 |
| A SET FOR DETERMING THE ABUNDANCE OF THE INSERTIONOF THE CONSTRUCT OF INTEREST INTO THE AAVS1LOCUS OF THE HUMAN GENOME |
2021 |
- Ivanenko Aleksandr Vyacheslavovich
- Belousov Vsevolod Vadimovich
|
RU2786396C1 |
| GENOMIC DNA EDITING SYSTEM OF AN EUKARYOTIC CELL BASED ON THE NUCLEOTIDE SEQUENCE ENCODING THE SUCAS9NLS PROTEIN |
2022 |
- Severinov, Konstantin Viktorovich
- Khodorkovskii, Mikhail Alekseevich
- Selkova, Polina Anatolevna
- Vasileva, Aleksandra Andreevna
- Abramova, Marina Viktorovna
- Arseniev, Anatolii Nikolaevich
- Chernova, Veronika Evgenevna
- Musharova, Olga Sergeevna
- Klimuk, Evgenii Ivanovich
|
RU2804422C1 |
| DELIVERY AND USE OF SYSTEMS OF CRISPR-CAS, VECTORS AND COMPOSITIONS FOR TARGETED ACTION AND THERAPY IN LIVER |
2014 |
- Chzhan Fen
- Tsun Le
- Zhan Fej
|
RU2716420C2 |
| CRISPR/CAS9 TYPE II GENOME EDITING SYSTEM AND ITS USE |
2022 |
- Tian Rui
- Huang Long
- Xie Hongxian
|
RU2794774C1 |
| PCDEXO-GS-SSB GENETIC CONSTRUCTS CONTAINING SEQUENCES ENCODING CHIMERIC SSB PROTEINS AND EXONUCLEASES OF THE BACTERIOPHAGE HOMOLOGOUS RECOMBINATION APPARATUS, COMBINED VIA A GLYCINE-SERINE POLYLINKER |
2020 |
- Kolosov Petr Mikhajlovich
- Pustogarov Nikolaj Andreevich
- Alkalaeva Elena Zinovevna
|
RU2763747C1 |
| RNA-CONDUCTOR St10 FOR USE IN THE HIGHLY SPECIFIC STREPTOCOCCUS THERMOPHILUS CRISPR / Cas9 (StCas9) NUCLEASES SYSTEM AND USE OF SAID RNA-CONDUCTOR AND StCas9 PROTEIN TO SUPPRESS HEPATITIS B VIRUS EXPRESSION IN THE HOST CELL AND FOR VIRAL DNA ELIMINATION FROM THE HOST CELL |
2018 |
- Kostyushev Dmitrij Sergeevich
- Kostyusheva Anastasiya Pavlovna
- Chulanov Vladimir Petrovich
- Chulanov Vladimir Petrovich
|
RU2694396C1 |
