CONJUGATE OF NANOPARTICLES OF GOLD-MAGNETITE COMPOSITION WITH FUNCTIONAL MOLECULE (OPTIONS) AND METHOD OF USE (OPTIONS) Russian patent published in 2024 - IPC C07F19/00 A61K47/50 A61K49/00 G01N33/53 B82B1/00 B82Y5/00 B82Y40/00 C07F1/12 C07F15/02 

FIELD: biomedicine and nanomedicine.

SUBSTANCE: invention relates to conjugates of a nanoparticle with a functional molecule for medical use, characterized by the following: on the surface of a water-compatible stable nanoparticle with magnetic properties consisting of gold and magnetite Fe₃O₄, a stabilizing compound with a carboxyl group is immobilized, which is a carbene ligand of formula (I), where R₁ and R₂ are independently selected from the group consisting of COOR₅ or COOR₆; R₃ and R₄ are independently selected from the group consisting of H, C₁-C₆ alkyl, C₃-C₇ cycloalkyl, C₁-C₆ alkyloxy and halogen; R₅ and R₆ are independently selected from the group consisting of H; C₁-C₆ alkyl; C₃-C₇ cycloalkyl; C₁-C₆ alkyloxy and halogen. The proposed conjugate retains colloidal stability after drying and has the ability to resuspend in water, in a buffer aqueous solution or in a biological sample. Nanoparticle sizes range from 5 to 100 nm. The specified nanoparticle is connected to the functional molecule directly or using a linker having the general formula СONH(CH₂)_m Y, where Y=COOH, NH₂, SH, CONH₂, OH, CHO, CO-PEG-OH or CO- PEI-NH₂, m can take values from 3 to 12, the functional molecule is selected from the group consisting of a therapeutic drug or a diagnostic substance. In particular, the functional group may be a small molecule, coumarin, photosensitizer, protein, carbohydrate, lipid, DNA, transporter, template, ribosomal or microRNA, synthetic nucleic acid analogs, synthetic polymer polyethylene glycol or polyethyleneimine, a pharmacologically active compound. The invention also relates to a method of using the specified conjugate in magnetic resonance imaging, spectrophotometric analysis, enzyme-linked immunosorbent assay, immunochromatographic analysis, as a contrast for computed tomography, for detection of target molecules, for delivery of target molecules into living cells and organisms, as an agent for radiotherapy, for targeted delivery of a therapeutic drug into cells.

EFFECT: increase of the colloidal stability of conjugates of magnetite-gold nanoparticles (Fe₃O₄@AuNP@NHC) with functional molecules to external physical and chemical exposure, including in biological environments, as well as facilitating the process of storage and transportation due to the ability of these conjugates to maintain colloidal stability upon drying, ensuring the possibility of delivering diagnostic and medicinal substances to target cells.

77 cl, 10 dwg, 29 ex