NANOPARTICLE OF GOLD-MAGNETITE COMPOSITION, METHOD OF PRODUCING NANOPARTICLE (VERSIONS), CONJUGATE BASED ON IT (VERSIONS), METHOD OF PRODUCING CONJUGATE AND TEST KIT Russian patent published in 2024 - IPC C07F19/00 A61K47/50 A61K49/00 G01N33/53 B82B1/00 B82Y5/00 B82Y40/00 C07F1/12 C07F15/02 

FIELD: biomedicine.

SUBSTANCE: invention relates to biomedicine, nanomedicine, particularly to a nanoparticle for biological and medical use. Nanoparticle is a water-compatible stable nanoparticle with magnetic properties, consisting of gold and magnetite Fe₃O₄ with a stabilizing compound with a carboxyl group immobilized on the surface of the nanoparticle. Compound is a carbene ligand of formula (I), where R₁ and R₂ are independently selected from the group consisting of COOR₅ or COOR₆; R₃ and R₄ are independently selected from a group comprising H, or C₁-C₆ alkyl, or C₃-C₇ cycloalkyl, or C₁-C₆ alkyloxy and halogen, or C₁-C₆ alkyl, C₃-C₇ cycloalkyl, or C₁-C₆ alkyloxy and halogen; R₅ and R₆ are independently selected from the group consisting of H, or C₁-C₆ alkyl, or C₃-C₇ cycloalkyl, or C₁-C₆ alkyloxy and halogen; n = 3. Nanoparticle is characterized by that it retains colloidal stability after drying and is capable of resuspension in water, in a buffer aqueous solution or a biological sample. Sizes of nanoparticles are in range from 5 to 100 nm. Invention also relates to a method of producing a nanoparticle, a method of producing a conjugate of a nanoparticle and a functional molecule, a conjugate of a nanoparticle and various functional molecules, a test kit for biological and medical use, in which said nanoparticle is used.

EFFECT: increased colloidal resistance of nanoparticles of composition magnetite-gold (Fe3O4@AuNP@NHC) to external physical and chemical effects, including in biological media, as well as facilitating the storage and transportation process due to the ability of said nanoparticles to preserve colloidal stability during drying.

67 cl, 10 dwg, 28 ex

(I)