ADVANCED CELL CULTURE DEVICE Russian patent published in 2024 - IPC C12M1/00 C12M3/00 C12M1/32 C12M1/12 

FIELD: laboratory equipment.

SUBSTANCE: group of inventions relates to a device for culturing cells and a method of its use. The cells are three-dimensional organotypic cells or tissues - spheroids. To obtain individual spheroid(s) on the discontinuous surface of the well, transfer the individual spheroid(s) to the well of the cell culture device. The well of the cell culture device contains a base and side walls extending from the base and delimiting the volume of the well. At the base or side walls of the well, there is an inlet port configured to transfer fluid into the well and an outlet port configured to transfer fluid from the well. The base of the well has a discontinuous surface configured to trap one or more individual spheroids within the discontinuous surface to reduce or prevent their agglomeration. The individual spheroid(s) are then brought into contact with a discontinuous surface and incubated in the well.

EFFECT: reduction or prevention of agglomeration of spheroids.

14 cl, 12 dwg