FIELD: biotechnology.
SUBSTANCE: described is a method for detecting the Henipavirus hendraense virus using the DETECTR method, involving extraction of RNA from biological samples followed by detection of target RNA fragments of the viral genome. Method comprises performing isothermal recombinase polymerase amplification with reverse transcription, combined in one test tube with fluorescent detection, by preliminary spatial separation of reaction mixtures for amplification of nucleic acids and fluorescent detection of DETECTR in a test tube with their subsequent mixing, using LbaCas12a nuclease, primers and probes.
EFFECT: wider range of products used for diagnosis, control and prevention of possible future epidemics, providing reliable diagnosis of the presence of RNA of Henipavirus hendraense virus.
1 cl, 1 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING MAMMARENAVIRUS MACHUPOENSE BY DETECTR METHOD WITH ISOTHERMAL AMPLIFICATION | 2024 |
|
RU2832917C1 |
| METHOD FOR DETECTING RNA OF HENIPAVIRUS HENDRAENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION | 2024 |
|
RU2834950C1 |
| METHOD FOR DETECTING RNA OF MAMMARENAVIRUS GUANARITOENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION | 2024 |
|
RU2834909C1 |
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| METHOD OF DETECTING NIPAH VIRUS BY METHOD BASED ON USE OF DEOXYRIBOZYME 10-23 | 2023 |
|
RU2816271C1 |
| METHOD FOR DETECTING BANDAVIRUS DABIEENSE (SFTSV) VIRUS RNA BY REAL-TIME RT-PCR | 2024 |
|
RU2831410C1 |
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD OF DETECTING NIPAH VIRUS USING REAL-TIME RT-PCR | 2022 |
|
RU2816270C2 |
| METHOD OF DETECTING RNA OF VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER USING REAL-TIME RT-PCR | 2024 |
|
RU2834908C1 |
