FIELD: medicine.
SUBSTANCE: invention relates to medical ecology, and can be used for differential assessment of the prevailing type of combined aerogenic effect of chemicals: aluminum oxide, benz(a)pyrene and hydrofluoride, on health of children 4–7 years old. In children living on territory with constant presence and/or with higher, in comparison with maximum permissible concentration, in atmospheric air of chemical substances: aluminum oxide, benz(a)pyrene and hydrofluoride, urine and blood samples are taken. Concentration of aluminum and fluoride-ion in urine, benz(a)pyrene in blood is determined in samples. If observing the concentration of exposure biomarkers in the child, namely: the content of aluminum in the urine of child is 0.010–0.012 mg/dm3 and fluoride ion 0.55–0.83 mg/dm3, and benz(a)pyrene in child’s blood (0.006–0.008)·10−3 mg/dm3, laboratory values are determined in the child's biological media, which are biomarkers of negative effects, which are pathogenetically related to the most probable negative effects—health disorders in children, on the part of critical organs and systems, which occur in response to exposure to chemicals: aluminum oxide—nervous system and respiratory organs; benz(a)pyrene—immune system; hydrofluoride—bone system and respiratory organs, namely: on the part of the immune system—total immunoglobulin G (IgG) content, IU/cm3; immunoglobulin G specific (IgGspec.) to benz(a)pyrene, relative units; phagocytic number, relative units, and phagocytic index in blood, relative units; on the part of the bone system—an indicator of tartrate-resistant acid phosphatase in blood serum, units/dm3; on the part of the nervous system—the level of neuron-specific enolase in blood serum, mcg/dm3; on the part of respiratory organs—level of total immunoglobulin E (IgE), g/dm3, and level of malondialdehyde (MDA), mcmol/cm3, in blood plasma. If the child’s laboratory values are within the quantitative range of laboratory values of the above biomarkers of negative effects, namely: on the part of the immune system: level of IgG 12.98–15.30 g/dm3, IgGspec. to benz(a)pyrene 0.11–0.214 relative units, phagocytic number 0.83–0.95 relative units and phagocytic index 1.70–1.81 relative units; bone system: content of tartrate-resistant acid phosphatase 2.53–3.09 units/dm3; nervous system: level of neuron-specific enolase 4.09–5.59 mcg/dm3; respiratory organs: level of IgE 58.99–196.63 IU/cm3 and MDA 2.37–2.61 mcmol/cm3, which are pathogenetically related to the effect of the above exposure biomarkers on the health of children, such biomarkers are defined as pathogenetically significant detected biomarkers of negative effects for a child. Determining the probability p of each detected biomarker of negative effects by formula: 
, where p is the probability of the child’s body response for each detected pathogenetically significant biomarker of negative effects with simultaneous development of the effects of the isolated and combined action of the three chemicals, with their binary combination; x1, x2, x3—concentration of aluminum in child’s urine, benz(a)pyrene in child’s blood and fluoride ion in child’s urine, respectively, during exposure to aluminum oxide, benz(a)pyrene and hydrofluoride, mg/dm3; b0 is the regression coefficient given in table 2 of the description, which characterizes the level of probability of the child’s body response for each detected pathogenetically significant biomarker of negative effects, regardless of the effect of the studied exposure markers—the background level; b1, b2, b3, b12, b13, b23 are regression coefficients given in table 2 of the description, characterizing the level of probability of the response of the child's body for each detected pathogenetically significant biomarker of negative effects, with isolated action of aluminum oxide, benz(a)pyrene and hydrofluoride, as well as with their combined action in a binary mixture. Determining the probability p1 of each detected pathogenetically significant biomarker of negative effects by formula: p1 = 
, where p1 is the probability of the child’s body response for each detected pathogenetically significant biomarker of negative effects, with simultaneous development of effects of aluminum oxide, benz(a)pyrene and hydrofluoride with their isolated action. An additional probability Δp of the detected pathogenetically significant biomarker of negative effects is determined by formula: Δp = p−p1, where Δp is the additional probability of the child’s body response for each detected pathogenetically significant biomarker of negative effects as a result of the effects of the combined action of aluminum oxide, benz(a)pyrene and hydrofluoride. For the identified biomarker of negative effects, with a positive value of Δp, the prevailing type of the combined aerogenic effect of aluminum, benz(a)pyrene and hydrofluoride on the health disorder of 4–7 year old child is considered to be synergetic, exceeding the sum of the negative effects of each individual substance, and with a negative value of Δp—as antagonistic, less than the sum of negative effects of each individual substance.
EFFECT: method provides the possibility of informative and evidentiary substantiation and establishment of differential assessment of the type: synergetic or antagonistic, combined aerogenic simultaneous effect of aluminum oxide, benz(a)pyrene and hydrofluoride, on the health of children 4–7 years old, due to analysis of parameterised cause-and-effect relationships of exposure of influencing substances and negative effects.
1 cl, 10 tbl, 2 ex
