FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, molecular-genetic diagnostics, and can be used in medicine in determining the hereditary predisposition to the development of diseases associated with the carriage of the polymorphic variant rs1042665 (T>C) of the HSPA9 gene. Disclosed is a method for genotyping a polymorphic locus rs1042665 (T>C) of the HSPA9 gene in a human by real-time PCR using allele-specific fluorescent probes, which involves PCR using specially selected primers (forward 5'-AAATAAGCTCCGGCTGAAAA-3' and reverse 5'-GACAGGGGTTGATTTGACTAAA-3') and probes with fluorophores (T-allele-specific fluorescent-labelled probe 5'-(FAM)AGTTCACATTTAGCCT(RT'-(ROX)AGTTCACACTTAGCCT(BHQ2)-3') in an amplifier with fluorescence detection.
EFFECT: invention widens the range of methods for genotyping polymorphic variants of the HSPA9 gene, is characterized by simplicity, accuracy and low cost.
1 cl, 1 dwg
