FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to a method for stimulating production of neurotrophic growth factors by neuroglial cells in vitro. Problem solved by the present invention is to increase the effectiveness of the method and expand the range of effective methods for stimulating the production of neurotrophic growth factors by neuroglial cells in vitro. Task is achieved by the fact that in vitro in the culture medium MACS Neuro Medium with the addition of PI3K inhibitors: LY294002 or CL27c, unfractionated cells of the subventricular zone of the cerebral hemispheres are cultured in a CO2 incubator at 37 °C, 5% CO2 and 100% air humidity.
EFFECT: proposed method enables to effectively stimulate production of neurotrophic growth factors by neuroglial cells in vitro.
1 cl, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR STIMULATING THE PRODUCTION OF NEUROTROPHIC GROWTH FACTORS BY NEUROGLIAL CELLS IN VITRO | 2022 |
|
RU2793910C1 |
| METHOD FOR STIMULATING GRANULOCYTE COLONY STIMULATING FACTOR PRODUCTION BY BONE MARROW CELLS IN VITRO | 2017 |
|
RU2665818C1 |
| METHOD FOR STIMULATING PRODUCTION OF ERYTHROPOETIN BY BONE MARROW CELLS IN VITRO | 2016 |
|
RU2628882C1 |
| METHOD FOR STIMULATION OF GRANULOCYTE COLONY-STIMULATING FACTOR PRODUCTION BY BONE MARROW CELLS IN VITRO | 2020 |
|
RU2741784C1 |
| METHOD FOR STIMULATING PRODUCTION OF GRANULOCYTE COLONY-STIMULATING FACTOR BY BONE MARROW CELLS IN VITRO | 2021 |
|
RU2770784C1 |
| METHOD FOR STIMULATION OF ERYTHROPOIETIN PRODUCTION BY BONE MARROW CELLS IN VITRO | 2019 |
|
RU2713122C1 |
| HEMOSTIMULATING AGENT | 2017 |
|
RU2647833C1 |
| TISSUE REGENERATION STIMULATING AGENT | 2013 |
|
RU2599289C2 |
| METHOD FOR STIMULATING PRODUCTION OF ERYTHROPOIETIN IN A CELL CULTURE in vitro | 2015 |
|
RU2589288C1 |
| AGENT FOR CORRECTION OF MNESTIC DISORDERS | 2023 |
|
RU2834960C2 |
