FIELD: biotechnology; medicine.
SUBSTANCE: disclosed is a method of detecting Guanarito virus by a method based on the use of deoxyribozyme 10-23. RNA is extracted from biological samples, followed by detection of target RNA fragments of the Guanarito virus genome using oligonucleotide sequences of a biosensor based on deoxyribozyme 10-23 and a fluorescent-labelled probe, complementary to a specific fragment of the L segment of the Guanarito virus.
EFFECT: method enables reliable diagnosis of Guanarito virus RNA.
1 cl, 2 dwg, 1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTING NIPAH VIRUS BY METHOD BASED ON USE OF DEOXYRIBOZYME 10-23 | 2023 |
|
RU2816271C1 |
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD FOR DETECTING BANDAVIRUS DABIEENSE (SFTSV) VIRUS RNA BY REAL-TIME RT-PCR | 2024 |
|
RU2831410C1 |
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| METHOD OF PROGNOSTIC ASSESSMENT OF HEPATOTOXICITY IN HIV-INFECTED PATIENTS DURING ANTIRETROVIRAL THERAPY BASED ON THE DETERMINATION OF DELETION POLYMORPHISM OF GSTM1, GSTT1, CYP2D6 HUMAN XENOBIOTIC BIOTRANSFORMATION GENES AND A SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELED PROBES | 2022 |
|
RU2807530C1 |
| METHOD OF DETECTING MONKEY POX VIRUS OF MONKEYPOX TYPE BY REAL TIME PCR (MPX AMP PS) | 2023 |
|
RU2803898C1 |
| METHOD OF PROGNOSTIC ASSESSMENT OF DEVELOPMENT OF HEPATOCELLULAR CARCINOMA BASED ON DETERMINATION OF POLYMORPHISM OF HUMAN IFNAR-1 GENE AND A SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS | 2022 |
|
RU2811763C1 |
| METHOD FOR DETECTING RECOMBINANT FORMS OF HEPATITIS C VIRUS AND HEPATITIS C VIRUS DRUG RESISTANCE MUTATIONS TO DIRECT ANTIVIRAL DRUGS BY POLYMERASE CHAIN REACTION WITH SUBSEQUENT SEQUENCING | 2023 |
|
RU2824565C1 |
| METHOD FOR TICK-BORNE ENCEPHALITIS VIRUS DETECTION BY REAL-TIME RT-PCR METHOD | 2019 |
|
RU2744187C1 |
