METHOD OF NATIVE CELL RESEARCH Russian patent published in 2010 - IPC G01N33/49 

Abstract RU 2398234 C1

FIELD: medicine.

SUBSTANCE: blood sampling is performed, blood samples are prepared, cells are scanned in mode of semi-contact atomic-force microscopy and geometrical parametres of cells are measured. Sample is prepared from suspension of native cells, by application of drop onto clean degreased microscope slide, sample is placed into wet chamber, saturated with water vapour, closed with membrane and scanning in water vapour in semi-contact mode is performed with application of cantilever of NSG 03 series with stiffness 0.4 N/m, beam length 100 mcm and resonance frequency within 62-123 KHz.

EFFECT: application of method allows to reduce research time and obtain cell scans with high resolution, preserving their viability, native size and shape.

2 ex, 1 tbl, 2 dwg

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RU 2 398 234 C1

Authors

Fedorova Marina Zotovna

Chernjavskikh Svetlana Dmitrievna

Skorkina Marina Jur'Evna

Sladkova Evgenija Anatol'Evna

Zabinjakov Nikita Aleksandrovich

Dates

2010-08-27Published

2009-07-01Filed