FIELD: chemistry.
SUBSTANCE: invention relates to a nucleotide covalently attached to a fluorophore through a linker A, which can be used in nucleic acid analysis techniques, corresponding to formula:
,
where linker A has a structure of formula (I) or (II):
(I), 
(II),
each of R1 and R2 is methyl, m is 0, n is 1; R3 is -NH-C(=O)O-tert-butyl, R4 is hydrogen, X is CH2 and p is equal to 2; or R3 is -NH-C(=O)CH3, R4 is hydrogen, X is CH2 and p is equal to 2.
EFFECT: disclosed is a novel modified nucleotide and a kit based thereon to increase efficiency of nucleotide incorporation in sequencing by synthesis.
4 cl, 12 dwg, 2 tbl, 1 ex
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