METHOD FOR OPTIMAL ACCESS TO THE ABDOMINAL PORTION OF THE ESOPHAGUS OF IMMUNODEFICIENT MICE IN ORTHOTOPIC TRANSPLANTATION OF A HUMAN OESOPHAGEAL TUMOR FRAGMENT Russian patent published in 2019 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to experimental oncology, and concerns a method of orthotopic transplantation of a human oesophageal tumor fragment to immunodeficient mice for producing tumor models more accurately reflecting molecular, genetic and morphological features of human oesophageal cancer. Substance of the method consists in performing orthotopic transplantation of a human oesophageal tumor fragment with immunodeficient mice, involving human oesophageal cancer tissue transplantation on the pre-damaged esophagus wall in the abdominal cavity of the immunodeficiency Nu/J mouse. Access to abdominal esophagus is performed by layer-by-section incision of skin and tissue of abdominal wall of mouse for 25 mm, beginning of incision is located on costal arch at the point of intersection with it of line drawn from left shoulder to left hip of animal, end of incision is located in area of midline of animal and, entering it, line of incision is perpendicular to body axis of animal. Left portion of the liver is moved upwards with the liver edge at angle of 45° to animal body axis. Esophagus is incised, receding 3 mm upwards from the esophagus transition level into the stomach. Esophagus is incised at angle of 35° to axis of esophagus with length of incision not less than half of oesophageal diameter, in total, 3-4 such incisions of adventitial and muscular layers of esophagus are performed at distance of 1–1.5 mm from each other. Esophagus wall is sutured together by the ligature, which has previously been implanted with a fragment of the human oesophageal tumor, by drawing the needle perpendicular to the previously applied oesophageal incisions. Ligature is tightened by fixing the tumor to the esophagus wall. Stomach of the animal is stitched by applying first suture 2 mm higher than the esophagus to the stomach, the gastric wall below the esophagus is sutured into the stomach, the sutures are applied parallel to the axis of the stomach, applying a traction at the suture. Force vector is directed at angle 35° to animal body axis, bringing abdominal esophagus into wound. Ligature is fixed on stomach. Abdominal cavity is closed.

EFFECT: method enables optimal access to the abdominal esophagus of immunodeficient mice in orthotopic transplantation of a human oesophageal tumor fragment to produce tumor models, more accurately reflecting molecular, genetic and morphological features of human oesophageal cancer that will facilitate development of new therapeutic strategies in treating this oncopathology.

1 cl, 5 dwg