METHOD OF TRANSPLANTATION OF A FRAGMENT OF A HUMAN COLON TUMOR INTO THE DESCENDING COLON OF IMMUNODEFICIENT MICE Russian patent published in 2021 - IPC G09B23/28 A61B17/00 

FIELD: medicine, experimental oncology.

SUBSTANCE: invention relates to medicine, namely experimental oncology. Skin, muscles and tissues of the abdominal wall of the lumbosacral area of the back are scarified along the axis of body 3 mm away from the backbone. The 10-mm insection starts 7 mm away from the tailhead. As the operating wound is expanded using surgical forceps, the colon is being visualized. At 5 mm from the transition of the descending colon into the back gut, a 3-mm lengthwise section of the seromuscular layer of the colon is made. Using a suture, a 27-mm³ fragment of a human colon tumor is fixed above the section made earlier, the abdominal space wall and the mouse’s skin are sutured.

EFFECT: the method makes it possible to create an orthotopic patient-like model of descending colon cancer most accurately reproducing the characteristics of human descending colon tumor and necessary to carry out pre-clinical studies of the mechanisms of oncogenesis and new therapeutic agents and methods.

1 cl, 3 dwg, 1 ex