FIELD: medicine.
SUBSTANCE: invention relates to medicine, namely experimental oncology. A method for obtaining an orthotopic PDX model of human brain glioblastoma on immunodeficient Balb/c Nude mice for preclinical study of antitumor effects of cytostatic drugs includes anesthesia of mice, implantation of tumor material obtained from a patient after surgical resection of glioblastoma by dissecting the skin on the head of an immunodeficient mouse, retreating 2 mm from the point of interocular width. Next, a hole in the skull is formed with the help of boron, into which a tumor fragment with a volume of 8 mm3 is placed. Then a Tachocomb is applied over the defect of the skull bone. Next, the surgical wound is sutured. When the tumor material reaches a volume of 36 mm3, xenografts of glioblastoma of the human brain are transplanted up to the 5th passage.
EFFECT: invention makes it possible to conduct preclinical tests of cytostatic drugs using a model that preserves certain histological characteristics of tumor material obtained from a donor patient and has a similar level of proliferative activity of tumor tissue.
1 cl, 2 dwg, 1 tbl
| Title | Year | Author | Number |
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| METHOD OF PRODUCTION OF SUBDERMAL XENOGRAFTS OF CELL LINE OF PEOPLE SKIN MELANOMA mel Cher WITH MUTATION V600E BRAF FOR PRECLINICAL STUDY OF ANTINEOPLASTIC TARGETED AGENTS | 2014 |
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