METHOD FOR OBTAINING PARTICLES BASED ON HEMATITE FOR THE DELIVERY OF GENETIC CONSTRUCTS INTO A CELL Russian patent published in 2022 - IPC A61K33/08 A61K33/26 

FIELD: biomedicine and nanomedicine.

SUBSTANCE: invention relates to the field of biomedicine and nanomedicine, in particular to a method for obtaining a hematite-based particle for transfection of a nucleic acid into a cell and a method for delivering a nucleic acid into a cell using said particle. To implement this production method, ferrihydrite particles are first mixed with a solution of an acid selected from sulfuric, nitric, hydrochloric, formic, phosphoric and acetic. In this case, the acid is concentrated, and its concentration after mixing with ferrihydrite particles is at least 50 wt.%. Next, the resulting mixture is incubated at a temperature of 0 to +4 degrees Celsius in order to form hematite particles. Then these hematite particles are transferred into an aqueous solution with pH of more than 2 in order to obtain a suspension. After that, a solution of a positively charged polymer and a solution of nucleic acid are added to the suspension of said hematite particles.

EFFECT: present invention makes it possible to obtain non-toxic hematite nanoparticles of controlled size in combination with genetic constructs for transfection of cells using readily available reagents in a manner with the possibility of easily scalable synthesis conditions.

38 cl, 7 dwg, 14 ex